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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for MR1-ligand cross-linking to discover metabolite antigens by proteomics
doi: 10.1016/j.xpro.2026.104399
Figure Lengend Snippet: Culture for growth, lysis and MR1-enrichment from A549 cells (A) A549 MR1 KO cells expressing either scMR1-WT or scMR1-K43A are cultured over 2-3 weeks until high numbers of cells (>250 × 10 6 ) are reached. (B) highlights the key steps of the lysis process in order to isolate the scMR1 molecules. (C) describes the basics of Strep-Tactin® enrichment, using a FPLC machine with Strep-Tactin® XT 4flow® columns. Results are expected as a clear peak on a chromatography trace. Fractions containing the peak will be loaded and run on an SDS-PAGE gel for size confirmation of scMR1 molecules.
Article Snippet:
Techniques: Lysis, Expressing, Cell Culture, Chromatography, SDS Page
Journal: Cell
Article Title: FAMIN Is a Multifunctional Purine Enzyme Enabling the Purine Nucleotide Cycle
doi: 10.1016/j.cell.2019.12.017
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Recombinant, CyQUANT Assay, Proliferation Assay, Membrane, Transfection, Bicinchoninic Acid Protein Assay, Software, Mutagenesis, Plasmid Preparation, Protease Inhibitor